解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A new method for detecting viral antibodies in cerebrospinal fluid is described. The technique has many advantages over previously published methods in that it is highly sensitive eliminating the need to concentrate the CSF, takes 5 h to complete, avoids the use of radionucleides, and most importantly circumvents prob...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122226
更新日期:1984-06-01 00:00:00
abstract::Four days of fasting in the rat reduced brown-adipose-tissue (BAT) mass, mitochondrial protein, and tissue protein content. Specific binding of guanosine diposphate (GDP) to BAT mitochondria was depressed by 55% in 4d-fasted rats. Rats fasted for 3 d, and then refed a single carbohydrate meal (40 kJ), showed a signifi...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01140499
更新日期:1984-04-01 00:00:00
abstract::Muscle tissue levels of d1-alpha-tocopherol (vitamin E) were significantly lower in endurance-trained rats than in sedentary animals, whether the animals were fed on vitamin-E-deficient or control (vitamin-E-sufficient) diets. In vitamin-E-deficient rats, liver tissue levels of vitamin E were significantly lower in th...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119661
更新日期:1984-03-01 00:00:00
abstract::Two mouse monoclonal antibodies against the human complement control protein, Factor H (beta 1H), are described. The antibodies are both IgG - gamma 1 - subclass and are directed against different epitopes on the human Factor H molecule. One of the antibodies, MRC OX 24, increases the cofactor activity of Factor H in ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01120205
更新日期:1983-12-01 00:00:00
abstract::When the amino-acid sequence of the 108-residue, rat tumour calcium-binding protein, oncomodulin, was aligned with that of rat muscle parvalbumin, 55 homologous positions were found, with an additional 33 single base-pair substitutions. This extensive homology, with virtual identity of the calcium-binding domains, sig...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121034
更新日期:1983-11-01 00:00:00
abstract::The cytosolic Ca2+ activity of insulin-releasing clonal cells (RINm5F) was studied with the intracellular fluorescent indicator quin-2. When the extracellular Ca2+ concentration was 1 mM, the basal cytosolic Ca2+ activity was 101 +/- 5 nM. Depolarization with 25 mM K+ increased this Ca2+ activity to at least 318 nM, a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01140663
更新日期:1983-10-01 00:00:00
abstract::Using both immunohistological study and photoaffinity labelling with radioactive azido-ATP, evidence is presented that the mitochondrial membranes of the brown adipose tissue of the human adult contain a 32 000-Mr uncoupling protein, which is probably similar to the uncoupling protein of BAT of rodents. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01120989
更新日期:1983-08-01 00:00:00
abstract::The concentration of Na+ and K+ and the pH in the cytoplasm of Lettré cells was measured by monitoring the net flux of H+, Na+, or K+ across the plasma membrane which had been rendered permeable to these ions by the action of Sendai virus. Ion flux was measured directly by analysis of cell composition, or indirectly b...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01172873
更新日期:1983-07-01 00:00:00
abstract::Capacity to synthesize glucose, urea, and ketone bodies is well maintained in hepatocytes after storage for at least 24 h at 4 degrees C. Substrates and albumin are the only requirements. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01120697
更新日期:1983-06-01 00:00:00
abstract::The concentration of carbonic anhydrase III (CAIII) in male rat liver was found to be 30 times greater than that in the female. Castration of male rats led to marked reduction in liver CAIII concentrations which could be partially restored to control levels by testosterone replacement. Marked developmental and senesce...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121959
更新日期:1983-05-01 00:00:00
abstract::Matrix-bound monoclonal antibodies against urokinase have been used to purify this enzyme by affinity chromatography. In a single-step procedure, urokinase can be isolated from crude preparations with high yield and high purity, and without loss of enzymatic activity. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122902
更新日期:1983-04-01 00:00:00
abstract::125I-insulin was shown to be internalized in vivo to a discrete population of low-density membranes (ligandosomes), distinct from the +Golgi, endoplasmic reticulum, plasma membrane, and lysosomes. However, analytical subcellular fractionation shows that glutathione-insulin transhydrogenase is localized to the endoplas...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122896
更新日期:1983-04-01 00:00:00
abstract::Fatty-acid synthesis has been measured in vivo with 3H2O in cafeteria-fed rats exhibiting diet-induced thermogenesis. Synthesis was decreased in brown adipose tissue, the liver, white adipose tissue, and the carcass of the cafeteria-fed animals compared to rats fed the normal stock diet. Whole-body synthesis was also ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122453
更新日期:1983-03-01 00:00:00
abstract::Transfer of Bomirski amelanotic melanoma cells from in vivo to in vitro growth conditions results in occurrence of rapid melanization in their cytoplasm. The melanized cells from primary cell culture initiate tumours in hamsters, which do not contain traces of melanin and resemble typical amelanotic melanoma. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121951
更新日期:1983-02-01 00:00:00
abstract::For improving Ostwald's viscometry, which is time-consuming and requires a relatively large volume of specimen to determine viscosity, we developed a capillary microviscometric method with an appropriate mathematical model, and have compared this method with Ostwald's method. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121952
更新日期:1983-02-01 00:00:00
abstract::Three genotypically suppressible alleles, a1X4, alcA125, and niaD500, are phenotypically suppressed by aminoglycoside antibiotics. Unsuppressible alleles at these loci are unaffected as are known missense mutations at the yA and gdhA loci. This is consistent with the premise that the suppressible mutations are nonsens...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121566
更新日期:1983-01-01 00:00:00
abstract::Heparin causes lysis of the multilamellar liposomes of all three charges, positive, neutral, and negative, and thus releases the entrapped [3H]glucose or chromate. The lytic effect of heparin is also observed in liposomes prepared from the lipids extracted from human red blood cells. Heparin is found to interact with ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121569
更新日期:1983-01-01 00:00:00
abstract::N-(2-Hydroxypropyl)methacrylamide copolymers bearing oligopeptide side-chains terminating in p-nitroaniline (NAp) were incubated with rat liver lysosomal enzymes in the presence of the thiol glutathione, and the rate of p-nitroaniline release was measured. Twelve of the 16 side-chains investigated were hydrolysed to r...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122173
更新日期:1982-12-01 00:00:00
abstract::Particulate membrane fractions from calf liver catalyze the release of glucose from GlcNAc2-Man9-Glc1-3-oligosaccharides. Maximal oligosaccharide-glucosidase activity was obtained at pH 6.2 and a detergent concentration of 0.5% Triton X-100. This activity could be distinguished from non-specific alpha-glucosidase acti...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114896
更新日期:1982-11-01 00:00:00
abstract::The principle of the dual inhibitor titration method for testing models of electron-transport phosphorylation is outlined, and the method is applied to the study of photophosphorylation in bacterial chromatophores. It is concluded that energy coupling is strictly localized in nature in this system, in the sense that f...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114933
更新日期:1982-10-01 00:00:00
abstract::31p nuclear magnetic resonance (NMR) spectra have been obtained from animal and human tumours grown in laboratory rodents. The tumour cells are only slightly more acid than the surrounding muscle but they tend to have large Pi and sugar phosphate peaks, suggesting anoxia, and large but variable phosphodiester peaks. T...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114835
更新日期:1982-09-01 00:00:00
abstract::An adenosine 3'5'-cyclic-monophosphate (Cyclic AMP)-dependent protein kinase has been identified and partially purified from the rat prostate tumor induced by 20-methylcholanthrene. This enzyme is stimulated 2- to 3-fold by the nucleotide. Equilibrium studies at pH 5.0 suggest the presence of a major class of binding ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114829
更新日期:1982-09-01 00:00:00
abstract::A slow phase of linear exocytosis by fibroblasts of previously endocytosed fluid, succeeding the initial burst of release, is described. This is not due to cell lysis, is virtually abolished at 4 degrees C, and is retarded when the lysosomal system is expanded. It may reflect membrane recycling. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01314215
更新日期:1982-08-01 00:00:00
abstract::The relative proportions of immunoregulatory T-cell subpopulations defined by OKT monoclonal antibodies are disturbed in chronic lymphocytic leukaemia (CLL) patients (even early mild cases) compared with normal subjects in the same age range. The mean ratio OKT4+:OKT8+ (helper:suppressor/cytotoxic) is reversed from th...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01314213
更新日期:1982-08-01 00:00:00
abstract::The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase ac...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115252
更新日期:1982-07-01 00:00:00
abstract::The increase in % phosphorylase a associated with muscle contraction is reversed after a few minutes despite continued contractile activity. The present study was undertaken to determine whether adrenaline can activate phosphorylase after deactivation has occurred during prolonged stimulation of contraction. We found ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01115118
更新日期:1982-05-01 00:00:00
abstract::Maclurin, the potent non-specific blood-group hemagglutinin present in extracts of Maclura pomifera, has been purified by a new biospecific affinity-chromatographic procedure. Additional studies have indicated that this hemagglutinin occurs as five closely related tetrameric protein isoforms derived from two non-coval...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01136724
更新日期:1982-04-01 00:00:00
abstract::The first demonstration of a method to incorporate radiolabeled methyl groups from [14C]formaldehyde into membrane proteins in human erythrocytes by reductive methylation in the presence of NaCNBH3 is described. This method is based on the work of Jentoft and Dearborn [(1979) J. Biol. Chem. 254, 4359-4365], who demons...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01136722
更新日期:1982-04-01 00:00:00
abstract::The condensing component of chicken liver fatty acid synthetase is inhibited by a sulfhydryl reagent, iodoacetamide, with a second-order rate constant of 0.23 M-1 sec-1 at pH 7.0 and 0 degrees. Complete inactivation requires the modification of approximately 8 -SH groups per dimer of the enzyme. Quantitation of the ex...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116383
更新日期:1982-03-01 00:00:00
abstract::Significant amounts of somatostatin-like immunoreactivity (SLI) were detected in the extract of a human catecholamine-secreting adrenal medullary tumour. After salt fractionation and reconstitution the major portion of SLI was purified by gel filtration and two HPLC steps; in all three systems it eluted in the positio...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116377
更新日期:1982-03-01 00:00:00
abstract::Radioactivity from [14C] adenosine was linearly incorporated into tissue nucleotides in perfused rat hearts. All the TCA-extractable 14C was confined to the purine nucleoside phosphates for up to 1 h of perfusion. Radioactivity was also incorporated linearly into the TCA-insoluble fraction, which by 40 min accounted f...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114819
更新日期:1981-11-01 00:00:00
abstract::The rate of efflux of L-glutamate from renal brush-border membrane vesicles was enhanced by Na+ and by extravesicular L-glutamate, but not by D-glutamate nor analogs of L-glutamate that do not share the Na+-L-glutamate co-transport system. These results suggest that efflux was mediated by the Na+-L-glutamate carrier. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116468
更新日期:1981-09-01 00:00:00
abstract::Immobilization of yeast mitochondrial RNA on nitrocellulose filters without prior alkali treatment revealed a low-MW RNA species (integral of 300 bases) which hybridizes specifically to the RNA coding strand of a DNA fragment BglII-HinfI at the 3' end of the COB-BOX gene. This RNA species (often a doublet) was found i...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116301
更新日期:1981-07-01 00:00:00
abstract::The sites and extents of bisulphite-mediated deamination of cytidine residues in HeLa-cell 5.8 S rRNA within the 5.8 S / 28 S rRNA complex have been determined. Comparison of these data with equivalent data for isolated ('unbound') 5.8 S rRNA suggests that the conformation of several cytidine residues, including inter...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116191
更新日期:1981-05-01 00:00:00
abstract::Brain cells dissociated from 15-day-old embryonic mice and grown in culture contain both cytosolic and nuclear receptors for L-3,5,3'-triiodothyronine (L-T3). KD values for L-T3 of 3.05 X 10(-9) M and 4.2 X 10(-9) M were determined with the cytosolic and nuclear receptors respectively. These cultured cells, which are ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114868
更新日期:1981-04-01 00:00:00
abstract::31P NMR studies on microorganisms have been carried out with the cells embedded in agarose gel. The novel use of the gel for the NMR studies has advantages over the usual liquid suspensions in terms of improved reproducibility of data and cell viability, with no net loss of spectral quality. Polyphosphate formation in...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117011
更新日期:1981-02-01 00:00:00
abstract::Evidence is presented in support of a pathway in skeletal muscle of glyconeogenesis (glycogen biosynthesis de novo) from L-glutamate and related amino acids involving the enzyme phosphoenolpyruvate carboxykinase (PEP CK). In the rat hemidiaphragm in vitro, not only did L-[U]-14C]glutamate exert a glycogen-sparing acti...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117013
更新日期:1981-02-01 00:00:00